FT-IR Studies Of Lipid/Protein Interaction In Biological Membranes

Richard Mendelsohn; Mark Jaworsky; Gloria Anderle

doi:10.1117/12.970749

20 December 1985 FT-IR Studies Of Lipid/Protein Interaction In Biological Membranes

Richard Mendelsohn, Mark Jaworsky, Gloria Anderle

Proceedings Volume 0553, Fourier and Computerized Infrared Spectroscopy; (1985) https://doi.org/10.1117/12.970749
Event: 1985 International Conference on Fourier and Computerized Infrared Spectroscopy, 1985, Ottawa, Canada

Abstract

FT-IR spectroscopy has been used to monitor phospholipid order and melting characteristics as well as protein secondary structure in reconstituted membrane systems. The protein used in the current work is CaATPase, a Calcium-pumping protein isolated from rabbit skeletal muscle. The protein is isolated, purified and reconstituted into phospholipid vesicles of the desired composition. High precision (0.03 cm-1) measurements of the temperature dependence of the acyl chain CH2 stretching frequencies are used to construct melting curves for the various phospholipids used in the current work. The effect of CaATPase on lipid order depends upon the level of unsaturation of the lipid acyl chains, the strongest perturbations being observed for molecules containing one saturated and one unsaturated chain. Use of acyl chain perdeuterated lipids as one component of a binary lipid mixture permits studies of the thermal response of each component in either binary lipid mixtures or ternary complexes composed of two lipids plus protein. The tendencies of this integral membrane protein to preferentially interact with a particular lipid component is noted in several instances.

Citation Download Citation

Richard Mendelsohn, Mark Jaworsky, and Gloria Anderle "FT-IR Studies Of Lipid/Protein Interaction In Biological Membranes", Proc. SPIE 0553, Fourier and Computerized Infrared Spectroscopy, (20 December 1985); https://doi.org/10.1117/12.970749

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