Laser-induced fluorescence imaging system for protein separations in microfluidic devices

Champak Das; Alexander Stoyanov; Carl Fredrickson; Roger Tran-Son-Tay; Zhonghui Hugh Fan

doi:10.1117/12.576887

8 December 2004 Laser-induced fluorescence imaging system for protein separations in microfluidic devices

Champak Das, Alexander Stoyanov, Carl Fredrickson, Roger Tran-Son-Tay, Zhonghui Hugh Fan

Proceedings Volume 5591, Lab-on-a-Chip: Platforms, Devices, and Applications; (2004) https://doi.org/10.1117/12.576887
Event: Optics East, 2004, Philadelphia, Pennsylvania, United States

Abstract

This paper describes a laser-induced fluorescence (LIF) detection system for imaging proteins separated in a microfluidic device. The diameter of a laser beam is first increased through a beam expander, and subsequently focused into a line using a cylindrical lens. The resultant laser line is used to image an entire capillary or channel in which protein separation took place. The fluorescence emission is collected with a cooled, scientific grade charge-coupled device (CCD) camera. The detection limit was determined using a series of concentrations of fluorescein solutions. The temporal and spatial effects of photobleaching from laser irradiation were analyzed and the parameters to reduce the effect of photobleaching are discussed. We used the imaging system to demonstrate rapid analysis of proteins using isoelectric focusing.

Citation Download Citation

Champak Das, Alexander Stoyanov, Carl Fredrickson, Roger Tran-Son-Tay, and Zhonghui Hugh Fan "Laser-induced fluorescence imaging system for protein separations in microfluidic devices", Proc. SPIE 5591, Lab-on-a-Chip: Platforms, Devices, and Applications, (8 December 2004); https://doi.org/10.1117/12.576887

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