Direct led-fluorescence method for Mao-B inactivation in the treatment of Parkinson's

Jimmy A. Castillo; Jannett Hung; M. Rodriguez; E. Bastidas; I. Laboren; A. Jaimes

doi:10.1117/12.590947

21 October 2004 Direct led-fluorescence method for Mao-B inactivation in the treatment of Parkinson's

Jimmy A. Castillo, Jannett Hung, M. Rodriguez, E. Bastidas, I. Laboren, A. Jaimes

Proceedings Volume 5622, 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications; (2004) https://doi.org/10.1117/12.590947
Event: 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications, 2004, Porlamar, Venezuela

Abstract

A led-fluorescence spectroscopy method determinate the inhibitory effects of probe compounds on MAO-B activity is described. In this assay, we demonstrate the possibility of determinate the activity of MAO-B efficiently and rapidly without the use of reference substrate. Measuring variations in fluorescence intensity of MAO-B enzyme during the reaction with inhibitors, L-deprenyl and berberine IC₅₀ and K_I values were obtained. For L-deprenyl (IC₅₀ = 0.017 μM and K_I = 0.019 μM) and berberine (IC₅₀ = 90 μM and K_I = 47 μM) were in agreement to the values obtained with a standard method and literature reported.

Citation Download Citation

Jimmy A. Castillo, Jannett Hung, M. Rodriguez, E. Bastidas, I. Laboren, and A. Jaimes "Direct led-fluorescence method for Mao-B inactivation in the treatment of Parkinson's", Proc. SPIE 5622, 5th Iberoamerican Meeting on Optics and 8th Latin American Meeting on Optics, Lasers, and Their Applications, (21 October 2004); https://doi.org/10.1117/12.590947

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