Study on ultraviolet-light-excited blood fluorescence spectra characteristics

Shumei Gao; Changde Peng; Ying Liu

doi:10.1117/12.571625

18 January 2005 Study on ultraviolet-light-excited blood fluorescence spectra characteristics

Shumei Gao, Changde Peng, Ying Liu

Proceedings Volume 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II; (2005) https://doi.org/10.1117/12.571625
Event: Photonics Asia, 2004, Beijing, China

Abstract

The ultraviolet light-excitated fluorescence spectra from healthy human blood in vitro have been measured by FLS920 Spectrometer, made in Edinburgh Instruments, exciting light from Xe900 - 450W steady state xenon lamp. The relation between exciting light wavelength and the fluorescence spectral characteristics of blood in the case of certain concentration is provided in this paper. Ultraviolet light excitated healthy human blood fluorescence spectra profiles change in peak intensity and position with exciting light. It may be due to the abruption of large numbers of anisomerous C-C bonds or C-N bonds that absorb the energy by non-resonance on membrane of blood cells, bringing lone-pairs of electrons, forming the new fluorophores and emit fluorescence in the case of later ultraviolet light excitation. It may be why ultraviolet light is able to kill and wound cells. In addition, the fluorescence spectra distributing range is very wide. It is due to contributions of the many fluorophores with large numbers of vibrational energy levels on the ground level in the blood cells. Our understanding of the different wavelength light-induced blood cells fluorescence spectra characteristics may be useful to development of low level laser therapy in vivo and in vitro.

Citation Download Citation

Shumei Gao, Changde Peng, and Ying Liu "Study on ultraviolet-light-excited blood fluorescence spectra characteristics", Proc. SPIE 5630, Optics in Health Care and Biomedical Optics: Diagnostics and Treatment II, (18 January 2005); https://doi.org/10.1117/12.571625

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available