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9 February 2005 Scanning probe microscope visualization of t-loop assembly by TRF2 in cells
En-Hua Cao, Xiao-Fe Guo, Ju-Jun Wang, Jing-Fen Qin
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Proceedings Volume 5635, Nanophotonics, Nanostructure, and Nanometrology; (2005) https://doi.org/10.1117/12.571839
Event: Photonics Asia, 2004, Beijing, China
Abstract
Telomeres are essential nucleoprotein structure at the ends of all eukaryotic chromosomes. Our previous work demonstrated that mammalian telomeres were shown to end in a large t-loop structure in vitro and the formation of t-loops was dependent on the presence of TRF2. In this work, the telomere DNA and its complex of TRF2 in HeLa cells has been direct observed in the nanometer resolution regime by atomic force microscopy (AFM) and scanning near-field optical microscopy (SNOM). AFM images showed that the looped structures exited in cell extract containing TRF2, but it disappeared in the protein-deleted samples. When cells were pretreated by UV light plus psoralen, the looped structure could be observed in the protein-deleted samples. SNOM images further demonstrated TRF2 and p53 proteins in cell was bound at the loop junction. Above results suggest that the telomere t-loop structure by TRF2 play a important role in cell-senescence, and might signals p53 protein directly through association with the t-loop junction in cells.
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En-Hua Cao, Xiao-Fe Guo, Ju-Jun Wang, and Jing-Fen Qin "Scanning probe microscope visualization of t-loop assembly by TRF2 in cells", Proc. SPIE 5635, Nanophotonics, Nanostructure, and Nanometrology, (9 February 2005); https://doi.org/10.1117/12.571839
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KEYWORDS
Proteins
Near field scanning optical microscopy
Atomic force microscopy
In vitro testing
Optical microscopy
Visualization
Cell death
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