Retinal damage following exposure to pulsed Nd:YAG laser radiation in rabbits and its relation to physical parameters

Rachel Brandeis; David Peri; Josef Turetz; Eliezer Fishbine; Inbal Egoz; Tamar Kadar

doi:10.1117/12.588567

18 April 2005 Retinal damage following exposure to pulsed Nd:YAG laser radiation in rabbits and its relation to physical parameters

Rachel Brandeis, David Peri, Josef Turetz, Eliezer Fishbine, Inbal Egoz, Tamar Kadar

Author Affiliations +

Proceedings Volume 5688, Ophthalmic Technologies XV; (2005) https://doi.org/10.1117/12.588567
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The aim of the present study was to characterize permissible exposure limits (MPE) for safety analysis, with an emphasis on the immediate retinal damage, following Nd:YAG Q-switched laser radiation, and to test its correlation to physical parameters. Pigmented rabbits were exposed to Nd:YAG laser radiation (532nm, pulse duration: 20ns) in various energies. Exposures were conducted in retina tissue, very close to the optic nerve, with a total of 20 exposures per retina. Retinas were viewed during the first 10 min following exposure, using an on-line digital video camera. Thereafter, animals were sacrificed for histological evaluation. A part of the retinas were evaluated 24 hours post exposure. A quantitative analysis of the clinical findings, based on a severity score scale and a morphometric analysis of the extent of the lesions, was used to test the statistical relationship with the laser energy and number of pulses. In addition, hemorrhage threshold values were computed using Probit Analysis. Retinal damage, at various levels of severity, was observed immediately after exposure to energies above 10μJ, characterized by edema and subretinal hemorrhages. The appearance and severity of the lesions varied among animals, between fellow eyes and even within the same retina. The relationship between severity and extent of lesions, and energy levels and number of pulses was evaluated. The ED50 for various, immediate types of hemorrhage was determined, and correlated to physical parameters. Histological observations strengthened the clinical findings. The results were discussed in accordance with photomechanical and thermal theories of laser-tissue interactions.

Citation Download Citation

Rachel Brandeis, David Peri, Josef Turetz, Eliezer Fishbine, Inbal Egoz, and Tamar Kadar "Retinal damage following exposure to pulsed Nd:YAG laser radiation in rabbits and its relation to physical parameters", Proc. SPIE 5688, Ophthalmic Technologies XV, (18 April 2005); https://doi.org/10.1117/12.588567

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