Characterizing cellular contractility and cytoplasmic flow using spectral domain phase microscopy

Audrey K. Ellerbee; Michael A. Choma; Emily J. McDowell; Anthony L. Creazzo; Joseph A. Izatt

doi:10.1117/12.592809

13 April 2005 Characterizing cellular contractility and cytoplasmic flow using spectral domain phase microscopy

Audrey K. Ellerbee, Michael A. Choma, Emily J. McDowell, Anthony L. Creazzo, Joseph A. Izatt

Author Affiliations +

Proceedings Volume 5690, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX; (2005) https://doi.org/10.1117/12.592809
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The phase information inherent to spectral domain optical coherence tomography (OCT) processing can be exploited to resolve sub-coherence length displacement variations. Spectral domain phase microscopy (SDPM) is a functional extension of Fourier domain OCT whose common-path topology enables extraordinary phase sensitivity. Here we demonstrate the usefulness of SDPM in three biologically relevant applications: real-time tracking of cell surface displacements of contracting cardiac myocytes, extracting cytoplasmic flow characteristics for a single-celled organism (flow rates ~10-30μm/s), and cellular mechanical responses to cytoskeletal drug treatments. The results of these experiments are corroborated by light microscopy acquired concurrently with the SDPM data.

Citation Download Citation

Audrey K. Ellerbee, Michael A. Choma, Emily J. McDowell, Anthony L. Creazzo, and Joseph A. Izatt "Characterizing cellular contractility and cytoplasmic flow using spectral domain phase microscopy", Proc. SPIE 5690, Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine IX, (13 April 2005); https://doi.org/10.1117/12.592809

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