Full-field time domain optical molecular imaging system

David J. Hall; David R. Vera; Robert F. Mattrey M.D.

doi:10.1117/12.590250

28 April 2005 Full-field time domain optical molecular imaging system

David J. Hall, David R. Vera, Robert F. Mattrey M.D.

Proceedings Volume 5693, Optical Tomography and Spectroscopy of Tissue VI; (2005) https://doi.org/10.1117/12.590250
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The advent of optical molecular probes has taken optical imaging beyond approaches limited to intrinsic optical contrast mechanisms. Fluorophores are typically used as the source of contrast for optical molecular probes and the field of optical molecular imaging is concerned with measuring and quantifying their in vivo biodistribution and pharmacokinetics. Most optical molecular imaging systems are based on Continuous Wave (CW) approaches which enable rapid, full-body imaging of small animals and readily yield images of probe location, however quantification of probe concentration is challenging. Time Domain (TD) approaches, although more expensive and complicated than CW, provide more information to assist in determining the probe location and concentration. Moreover, the TD approach permits access to measuring the fluorophore lifetime which can be indicative of the probe’s environment. Existing TD approaches involve a point source and detector which are sequentially scanned over the sample and can take several minutes to acquire the data compared to the rapid imaging offered by CW. The system presented here employs a high power, near infrared, pulsed laser to provide area illumination and a temporally gated intensified CCD camera to permit area detection in order to enable rapid, full-body, TD optical molecular imaging of small animals in vivo. The system is described and preliminary in vitro and in vivo measurements are presented.

Citation Download Citation

David J. Hall, David R. Vera, and Robert F. Mattrey M.D. "Full-field time domain optical molecular imaging system", Proc. SPIE 5693, Optical Tomography and Spectroscopy of Tissue VI, (28 April 2005); https://doi.org/10.1117/12.590250

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available