Confocal photothermal flow cytometry in vivo

Vladimir P. Zharov; Ekaterina I. Galanzha; Scott Ferguson; Valery V. Tuchin

doi:10.1117/12.595421

25 April 2005 Confocal photothermal flow cytometry in vivo

Vladimir P. Zharov, Ekaterina I. Galanzha, Scott Ferguson, Valery V. Tuchin

Proceedings Volume 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics; (2005) https://doi.org/10.1117/12.595421
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

The new experimental design of an integrated flow cytometry (FC) is presented, combining high-resolution transmission digital microscopy (TDM) with photothermal (PT), photoacoustic (PA), and fluorescence techniques. We used phantom in vitro to verify this concept with moving living cells, and micro- and nanoparticles. The transistion in vivo study was realized by using unique rat mesentery model for real-time detection of circulating red and white blood cells. The adaptation of confocal schematics to PT microscopy to provide 3-D measurement is discussed. We demonstrated that simulataneous transmission, PT and fluorescent imaging provide the basis for nanodiagnostics and nanotherpeutics in vivo with gold nanoparticles as PT probes and sensitizers as well as identification cells with specific absorbing endogenous and exogenous structures. First attempt to use in parallel PA methods with detection PA signals from single live cells are presented. Potential applications of integrated FC are discussed, including identification of selected cells with different natural absorptive properties, characterization of bioflow (e.g., velocity profile), and PT nanotherapeutics and nanodiagnostics of metastatic cells with gold nanoparticles.

Citation Download Citation

Vladimir P. Zharov, Ekaterina I. Galanzha, Scott Ferguson, and Valery V. Tuchin "Confocal photothermal flow cytometry in vivo", Proc. SPIE 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, (25 April 2005); https://doi.org/10.1117/12.595421

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