Optical and acoustical monitoring of femtosecond laser-induced intracellular contrast agents: initial cell culture studies

Marwa J. Zohdy; Christine Tse; Jing Yong Ye; Lajos P. Balogh; Theodore B. Norris; Matthew O'Donnell

doi:10.1117/12.589687

25 April 2005 Optical and acoustical monitoring of femtosecond laser-induced intracellular contrast agents: initial cell culture studies

Marwa J. Zohdy, Christine Tse, Jing Yong Ye, Lajos P. Balogh, Theodore B. Norris, Matthew O'Donnell

Author Affiliations +

Proceedings Volume 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics; (2005) https://doi.org/10.1117/12.589687
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Acoustical monitoring of laser-induced optical breakdown can be used as an important tool for diagnostics and therapeutics in living cells. Laser-induced intracellular microbubbles provide measurable contrast when detected with high-frequency ultrasound, and the bioeffects of these bubbles can be controlled to be within two distinct regimes. In the nondestructive regime, a single, transient, detectable bubble can be generated within a cell, without affecting its viability. In the destructive regime, the induced photodisruption can kill a target cell. To generate and monitor this range of effects in real time, we have developed a system integrating a femtosecond pulsed laser source with optical and acoustical microscopy. Experiments were performed on monolayers of Chinese hamster ovary cells. A Ti:Sapphire laser (793 nm wavelength, 100 fs pulse duration) was pulsed at 3.8 kHz and tightly focused to a 1 μm spot within each cell, and a high-frequency (50 MHz) ultrasonic transducer monitored the generated bubble with continuous pulse-echo recordings. The photodisruption was also observed with bright field optical microscopy, and cell viability was assessed after laser exposure using a colorimetric live/dead stain. By controlling laser pulse fluence, exposure duration, and the intracellular location of the laser focus, either nondestructive or destructive bubbles could be generated.

Citation Download Citation

Marwa J. Zohdy, Christine Tse, Jing Yong Ye, Lajos P. Balogh, Theodore B. Norris, and Matthew O'Donnell "Optical and acoustical monitoring of femtosecond laser-induced intracellular contrast agents: initial cell culture studies", Proc. SPIE 5697, Photons Plus Ultrasound: Imaging and Sensing 2005: The Sixth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acousto-optics, (25 April 2005); https://doi.org/10.1117/12.589687

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KEYWORDS

Acoustics

Nondestructive evaluation

Femtosecond phenomena

Optical microscopy

Transducers

Target detection

Laser optics

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