Dual-modality imaging in vivo with an NIR and gamma emitter using an intensified CCD camera and a conventional gamma camera

Jessica P. Houston; Shi Ke; Wei Wang; Chun Li; Eva M. Sevick-Muraca

doi:10.1117/12.590858

4 April 2005 Dual-modality imaging in vivo with an NIR and gamma emitter using an intensified CCD camera and a conventional gamma camera

Jessica P. Houston, Shi Ke, Wei Wang, Chun Li, Eva M. Sevick-Muraca

Author Affiliations +

Proceedings Volume 5704, Genetically Engineered and Optical Probes for Biomedical Applications III; (2005) https://doi.org/10.1117/12.590858
Event: SPIE BiOS, 2005, San Jose, CA, United States

Abstract

Fluorescence-enhanced optical imaging measurements and conventional gamma camera images on human M21 melanoma xenografts were acquired for a "dual-modality" molecular imaging study. The avb3 integrin cell surface receptors were imaged using a cyclic peptide, cyclopentapeptide cyclo(lys-Arg-Gly-Asp-phe) [c(KRGDf)] probe which is known to target the membrane receptor. The probe, dual-labeled with a radiotracer, 111Indium, for gamma scintigraphy as well as with a near-infrared dye, IRDye800, was injected into six nude mice at a dose equivalent to 90mCi of 111In and 5 nanomoles of near-infrared (NIR) dye. A 15 min gamma scan and 800 millisecond NIR-sensitive ICCD optical photograph were collected 24 hours after injection of the dual-labeled probe. The image quality between the nuclear and optical data was investigated with the results showing similar target-to-background ratios (TBR) based on the origin of fluorescence and gamma emissions at the targeted tumor site. Furthermore, an analysis of SNR versus contrast showed greater sensitivity of optical over nuclear imaging for the subcutaneous tumor targets measured by surface regions of interest.

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Jessica P. Houston, Shi Ke, Wei Wang, Chun Li, and Eva M. Sevick-Muraca "Dual-modality imaging in vivo with an NIR and gamma emitter using an intensified CCD camera and a conventional gamma camera", Proc. SPIE 5704, Genetically Engineered and Optical Probes for Biomedical Applications III, (4 April 2005); https://doi.org/10.1117/12.590858

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