3D in-vivo imaging of GFP-expressing T-cells in mice with non-contact fluorescence molecular tomography

Anikitos Garofalakis; Heiko Meyer; Giannis Zacharakis; Eleftherios N. Economou; Clio Mamalaki; Joseph Papamatheakis; Vasilis Ntziachristos; Jorge Ripoll

doi:10.1117/12.634830

22 June 2005 3D in-vivo imaging of GFP-expressing T-cells in mice with non-contact fluorescence molecular tomography (Invited Paper)

Anikitos Garofalakis, Heiko Meyer, Giannis Zacharakis, Eleftherios N. Economou, Clio Mamalaki, Joseph Papamatheakis, Vasilis Ntziachristos, Jorge Ripoll

Author Affiliations +

Proceedings Volume 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI; (2005) https://doi.org/10.1117/12.634830
Event: Saratov, Russia, 2004, Saratov, Russian Federation

Abstract

Optical imaging and tomography in tissues can facilitate the quantitative study of several important chromophores and fluorophores in-vivo. Due to this fact, there has been great interest in developing imaging systems offering quantitative information on the location and concentration of chromophores and fluorescent probes. In this study we present a novel imaging system that enables three dimensional (3D) imaging of fluorescent signals in bodies of arbitrary shapes in a non-contact geometry, in combination with a 3D surface reconstruction algorithm, which is appropriate for in-vivo small animal imaging of fluorescent probes. The system consists of a rotating sample holder and a lens coupled Charge Coupled Device (CCD) camera in combination with a fiber coupled laser scanning device. An Argon ion laser is used as the source and different filters are used for the detection of various fluorophores or fluorescing proteins. With this new setup a large measurements dataset can be achieved while the use of inversion models give a high capacity for quantitative 3D reconstruction of fluorochrome distributions as well as high spatial resolution. The system has already been tested in the observation of the distribution of Green Fluorescent Protein (GFP) expressing T-lymphocytes in order to study the function of the immune system in a murine model, which can then be related to the function of the human immune system.

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Anikitos Garofalakis, Heiko Meyer, Giannis Zacharakis, Eleftherios N. Economou, Clio Mamalaki, Joseph Papamatheakis, Vasilis Ntziachristos, and Jorge Ripoll "3D in-vivo imaging of GFP-expressing T-cells in mice with non-contact fluorescence molecular tomography (Invited Paper)", Proc. SPIE 5771, Saratov Fall Meeting 2004: Optical Technologies in Biophysics and Medicine VI, (22 June 2005); https://doi.org/10.1117/12.634830

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