Micro x-ray fluorescence as a high throughput screening method for metal chelating compounds

Edel M. Minogue V.D.M.; George J. Havrilla; Tammy P. Taylor; Anthony K. Burrell; Benjamin P. Warner

doi:10.1117/12.605260

3 June 2005 Micro x-ray fluorescence as a high throughput screening method for metal chelating compounds

Edel M. Minogue V.D.M., George J. Havrilla, Tammy P. Taylor, Anthony K. Burrell, Benjamin P. Warner

Proceedings Volume 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy; (2005) https://doi.org/10.1117/12.605260
Event: OPTO-Ireland, 2005, Dublin, Ireland

Abstract

Micro X-ray Fluorescence (MXRF) has proven to be a powerful tool in the rapid and quantitative means of screening oliogpeptides. MXRF is a non-destructive method of analysis, which can detect elemental composition of a sample by measuring its characteristic X-ray emission wavelengths or energies. An effective high throughput screening technique is described for the rapid screening of bead-based libraries by MXRF in order to identify suitable chelating agents that will bind metals found in radioactive dispersive devices. It is a sensitive technique which in conjunction with the wide range of chemistry inherent in peptide libraries (e.g. varying charge, length, hydrophobicity, aromaticity etc.), provides a rapid and quantitative means for screening chelator-ion binding. The method involves the selection of a suitable library of ligands; in this case it is a bead-based library of peptides. The library is exposed to the cation of interest and immobilized on to a microarray. The array is then analyzed by MXRF enabling rapid identification of chelating agents. This enables the screening of approximately 27,500 sequences per day. Initial experiments carried out successfully identified sequences that are selective for Co under certain binding conditions. This involved the screening of 8,400 sequences in adverse environmental conditions containing possible interferences (e.g. Ca, Fe, Al, Cs, Ir), which could be encountered in our application.

Citation Download Citation

Edel M. Minogue V.D.M., George J. Havrilla, Tammy P. Taylor, Anthony K. Burrell, and Benjamin P. Warner "Micro x-ray fluorescence as a high throughput screening method for metal chelating compounds", Proc. SPIE 5826, Opto-Ireland 2005: Optical Sensing and Spectroscopy, (3 June 2005); https://doi.org/10.1117/12.605260

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KEYWORDS

Metals

X-rays

X-ray fluorescence spectroscopy

Cobalt

Chemical elements

Chemistry

X-ray optics

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