Optical coherence tomography imaging and fluorescence spectroscopy of a novel rat model of ovarian cancer

Elizabeth Kanter; Ross Walker; Sam Marion; Patricia Hoyer; Jennifer K. Barton

doi:10.1117/12.632983

7 October 2005 Optical coherence tomography imaging and fluorescence spectroscopy of a novel rat model of ovarian cancer

Elizabeth Kanter, Ross Walker, Sam Marion, Patricia Hoyer, Jennifer K. Barton

Author Affiliations +

Proceedings Volume 5861, Optical Coherence Tomography and Coherence Techniques II; 58610P (2005) https://doi.org/10.1117/12.632983
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

Ovarian cancer is relatively rare but is the fifth leading cause of death from cancer in women. Little is known about the precursors and early stages of ovarian cancer partially due to the lack of a realistic animal model. A cohesive model that incorporates ovarian cancer induction into a menopausal rodent would be well suited for comprehensive studies of ovarian cancer, and non-destructive imaging would allow carcinogenesis to be followed. Optical Coherence Tomography (OCT) and Light-Induced Fluorescence (LIF) are minimally invasive optical modalities that allow both structural and biochemical changes to be noted. Rat ovaries were exposed to 4-vinylcyclohexene diepoxide (VCD) for 20 days in order to destroy the primordial follicles. Sutures coated with 7,12-dimethylbenz(a)anthracene (DMBA) were implanted in the right ovary, in order to produce epithelial based ovarian cancers. Rats were sacrificed at 1, 3, and 5 months and ovaries were harvested and imaged with a combined OCT/LIF system. Histology was preformed on the harvested ovaries and any pathology determined. OCT was able to visualize follicle loss and DMBA-induced abnormalities. LIF spectra were also different between cycling, follicle deplete, and DMBA-exposed ovaries. Overall this pilot study demonstrated the feasibility of both the animal model and optical imaging.

Citation Download Citation

Elizabeth Kanter, Ross Walker, Sam Marion, Patricia Hoyer, and Jennifer K. Barton "Optical coherence tomography imaging and fluorescence spectroscopy of a novel rat model of ovarian cancer", Proc. SPIE 5861, Optical Coherence Tomography and Coherence Techniques II, 58610P (7 October 2005); https://doi.org/10.1117/12.632983

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