Nerve regeneration in C. elegans after femtosecond laser axotomy

Mehmet Fatih Yanik; Hulusi Cinar; Hediye Nese Cinar; Andrew D. Chisholm; Yishi Jin; Adela Ben-Yakar

doi:10.1117/12.633081

26 August 2005 Nerve regeneration in C. elegans after femtosecond laser axotomy

Mehmet Fatih Yanik, Hulusi Cinar, Hediye Nese Cinar, Andrew D. Chisholm, Yishi Jin, Adela Ben-Yakar

Proceedings Volume 5863, Therapeutic Laser Applications and Laser-Tissue Interactions II; 58630C (2005) https://doi.org/10.1117/12.633081
Event: European Conferences on Biomedical Optics 2005, 2005, Munich, Germany

Abstract

We show sub-micron scale surgery with femtosecond lasers in a tiny living organism. By just cutting few nano-scale nerve connections inside the nematode C. elegans, we can stop the whole worm from moving backwards. This delicate axotomy keeps the surrounding of the severed axons un-damaged so that the axons can regrow back, and the worms recover and can move backwards again. These results demonstrate, for the first time, nerve regeneration in such a tiny organism, in its evolutionarily simplest form. The ability to perform precise sub-micron scale axotomy on such organisms provides tremendous research potential for rapid screening of drugs and discovery of new biomolecules affecting regeneration and development.

Citation Download Citation

Mehmet Fatih Yanik, Hulusi Cinar, Hediye Nese Cinar, Andrew D. Chisholm, Yishi Jin, and Adela Ben-Yakar "Nerve regeneration in C. elegans after femtosecond laser axotomy", Proc. SPIE 5863, Therapeutic Laser Applications and Laser-Tissue Interactions II, 58630C (26 August 2005); https://doi.org/10.1117/12.633081

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