Multimodal optical imaging of mouse Ischemic cortex

Phillip B. Jones; Hwa Kyuong Shin; Andrew K. Dunn; Bradley T. Hyman; David A. Boas; Michael A. Moskowitz; Cenk Ayata

doi:10.1117/12.630946

21 November 2005 Multimodal optical imaging of mouse Ischemic cortex

Phillip B. Jones, Hwa Kyuong Shin, Andrew K. Dunn, Bradley T. Hyman, David A. Boas, Michael A. Moskowitz, Cenk Ayata

Proceedings Volume 6009, Optical Methods in Drug Discovery and Development; 60090X (2005) https://doi.org/10.1117/12.630946
Event: Optics East 2005, 2005, Boston, MA, United States

Abstract

Real time investigation of cerebral blood flow (CBF), and oxy/deoxy hemoglobin volume (HbO,HbR) dynamics has been difficult until recently due to limited spatial and temporal resolution of techniques like laser Doppler flowmetry and MRI. This is especially true for studies of disease models in small animals, owing to the fine structure of the cerebral vasculature. The combination of laser speckle flowmetry (LSF) and multi-spectral reflectance imaging (MSRI) yields high resolution spatio-temporal maps of hemodynamic changes in response to events such as sensory stimuli or arterial occlusion. Ischemia was induced by distal occlusion of the medial cerebral artery (dMCAO). Rapid changes in CBF, HbO, and HbR during the acute phase were captured with high temporal and spatial resolution through the intact skull. Hemodynamic changes that were correlated with vasoconstrictive events, peri-infarct spreading depressions (PISD), were observed. These experiments demonstrate the utility of LSF and Multi-spectral reflectance imaging (MSRI) in mouse disease models.

Citation Download Citation

Phillip B. Jones, Hwa Kyuong Shin, Andrew K. Dunn, Bradley T. Hyman, David A. Boas, Michael A. Moskowitz, and Cenk Ayata "Multimodal optical imaging of mouse Ischemic cortex", Proc. SPIE 6009, Optical Methods in Drug Discovery and Development, 60090X (21 November 2005); https://doi.org/10.1117/12.630946

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KEYWORDS

Chromophores

Scattering

Ischemia

Reflectivity

Hemodynamics

Speckle

Blood

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