Construction of a Fourier-transform phase-modulation fluorometer

Hironobu Shibata; Tetsuo Iwata

doi:10.1117/12.666813

9 December 2005 Construction of a Fourier-transform phase-modulation fluorometer

Hironobu Shibata, Tetsuo Iwata

Proceedings Volume 6024, ICO20: Optical Devices and Instruments; 60240B (2005) https://doi.org/10.1117/12.666813
Event: ICO20:Optical Devices and Instruments, 2005, Changchun, China

Abstract

We have constructed a Fourier-transform phase-modulation fluorometer (FT-PMF) by which a fluorescence decay waveform can be obtained. In the FT-PMF, the modulation frequency of the excitation light source is swept continuously from a direct current (dc) to a high frequency f_max with a time duration T. The resultant fluorescence signal waveform is Fourier-transformed to obtain its amplitude and phase spectra. The ratio of the amplitude spectrum and the difference of the phase spectrum over those of the reference spectra that are obtained from a non-fluorescent material are calculated, respectively, and the pair of both spectral data is inverse-Fourier-transformed again to obtain the fluorescence decay waveform. The light source used was an ultraviolet light emitting- diode (UV LED) whose typical operating condition was f_max = 100 MHz and T = 10 μs. To demonstrate the performance of the FT-PMF, we carried out (1) measurement of a fluorescent decay waveform of YAG materials packed in a white LED, and (2) determination of fluorescence lifetime of 10 ppm quinine sulfate in 0.1N H₂SO₄.

Citation Download Citation

Hironobu Shibata and Tetsuo Iwata "Construction of a Fourier-transform phase-modulation fluorometer", Proc. SPIE 6024, ICO20: Optical Devices and Instruments, 60240B (9 December 2005); https://doi.org/10.1117/12.666813

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