Experimental evidence for deeper interrogation inside cardiac tissue using transillumination and a fluorescence absorber

Venkat K. Ramshesh; Stephen B. Knisley

doi:10.1117/12.647004

1 March 2006 Experimental evidence for deeper interrogation inside cardiac tissue using transillumination and a fluorescence absorber

Venkat K. Ramshesh, Stephen B. Knisley

Author Affiliations +

Proceedings Volume 6084, Optical Interactions with Tissue and Cells XVII; 60840Y (2006) https://doi.org/10.1117/12.647004
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Limited optical interrogation depth prevents studies of arrhythmias in 3D tissue. Our experiments with single photon excitation and computer simulations with single and two-photon excitation show that the addition of a fluorescence absorber can modify the region of tissue interrogated with transillumination. Experiments were performed with a pair of 0.1 cm thick rabbit cardiac slices. The slices were excited with 488 nm single photon laser excitation while light from opposite side was collected for transillumination. One slice was stained with di-4-ANEPPS while the other was not. In different measurements, both slices were also stained with the red light absorbing dye, blue 1. In our models fluorescent photons were launched from specific regions of the tissue to mimic staining of different layers with di-4-ANEPPS. In additional simulations the fluorescence absorption coefficient was increased 3-fold. In our experiments with the di-4-ANEPPS slice facing away from the laser, measured light intensity was 68% of the value found with di-4-ANEPPS slice facing the laser while it was 21% in the model. This reduction in intensity from the deeper layer became less pronounced after addition of the red absorber. Then with di-4-ANEPPS slice facing away from the laser the measured light intensity was 81% of the value found with di-4-ANEPPS slice facing the laser in experiments while it was 34% in the model. This indicates deepening of the interrogated region by addition of red absorbing dye. In computer simulations using two-photon excitation at 1064 nm, increasing the fluorescence absorption further deepend the interrogated region compared with one photon excitation at 488 nm.

Citation Download Citation

Venkat K. Ramshesh and Stephen B. Knisley "Experimental evidence for deeper interrogation inside cardiac tissue using transillumination and a fluorescence absorber", Proc. SPIE 6084, Optical Interactions with Tissue and Cells XVII, 60840Y (1 March 2006); https://doi.org/10.1117/12.647004

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
7 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Luminescence

Absorption

Tissue optics

Tissues

Photons

Computer simulations

Monte Carlo methods

Show All Keywords

Keywords/Phrases

Search In:

Publication Years