Scanning total internal reflection fluorescence imaging

A. M. Quirke; S. M. Ameer-Beg; M. Parsons; T. Ng; M. Irving; B. Vojnovic

doi:10.1117/12.648451

23 February 2006 Scanning total internal reflection fluorescence imaging

A. M. Quirke, S. M. Ameer-Beg, M. Parsons, T. Ng, M. Irving, B. Vojnovic

Proceedings Volume 6089, Multiphoton Microscopy in the Biomedical Sciences VI; 608907 (2006) https://doi.org/10.1117/12.648451
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Cell adhesion and focal complex formation require signalling complexes linking cell adhesion molecules to the cytoskeleton. To understand morphogenetic changes associated with tumour cell spreading, migration and tumour cell metastasis, the molecular mechanisms responsible for the regulation, formation and dissolution at the cell-extracellular matrix (ECM) interface need to be identified. In order to achieve this, an improved axial resolution is desirable. We report on the development of a multi-photon (MP) total internal reflection (TIR) fluorescence lifetime imaging (FLIM) system that allows the selective excitation of fluorophores, with such an improved axial resolution. Results from initial experiments are presented. High excitation efficiency is achieved by the use of a Nikon 1.45 NA TIRF objective using annular illumination.

Citation Download Citation

A. M. Quirke, S. M. Ameer-Beg, M. Parsons, T. Ng, M. Irving, and B. Vojnovic "Scanning total internal reflection fluorescence imaging", Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 608907 (23 February 2006); https://doi.org/10.1117/12.648451

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