Imaging melanin by two-photon absorption microscopy

Tong Ye; Gunay Yurtsever; Martin Fischer; John D. Simon; Warren S. Warren

doi:10.1117/12.646139

23 February 2006 Imaging melanin by two-photon absorption microscopy

Tong Ye, Gunay Yurtsever, Martin Fischer, John D. Simon, Warren S. Warren

Proceedings Volume 6089, Multiphoton Microscopy in the Biomedical Sciences VI; 60891X (2006) https://doi.org/10.1117/12.646139
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Multiphoton excitation fluorescence microscopy has proven to be a powerful method for non-invasive, in vivo, thick tissue imaging with molecular specificity. However, many important endogenous biomolecules do not fluoresce (NAD) or fluoresce with low efficiency (Melanin). In this report femtosecond pulse shaping methods are used to measure two-photon absorption (TPA) directly with very high sensitivity. Combining with the laser scanning microscope, this Two-photon Absorption Microscopy (TPAM) retains the penetration and localization advantages of two-photon fluorescence microscopy and permits direct observation of important endogenous molecular markers (melanin or hemoglobin) which are invisible in multiphoton fluorescence microscopy. We have demonstrated here for the first time that TPAM can successfully and more efficiently image melanoma cells and tissues and provide a good melanin contrast in optical sectioning of the melanoma lesions which are comparable to pathological histology. Combining with the two-photon fluorescence images acquired simultaneously, the distribution patterns of the melanocytes and their intratissue behavior could be studied without cutting the lesions from patients. TPAM will undoubtedly find the applications in the clinical diagnosis and biomedical research.

Citation Download Citation

Tong Ye, Gunay Yurtsever, Martin Fischer, John D. Simon, and Warren S. Warren "Imaging melanin by two-photon absorption microscopy", Proc. SPIE 6089, Multiphoton Microscopy in the Biomedical Sciences VI, 60891X (23 February 2006); https://doi.org/10.1117/12.646139

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available