Molecular differentiation of <i>Leishmania</i> protozoarium using CdS quantum dots as biolabels

Beate S. Santos; Patrícia M. A. de Farias; Frederico D. de Menezes; Erick L. Mariano; Ricardo de C. Ferreira; Selma Giorgio; Maira C. Bosetto; Diana C. Ayres; Paulo M. Lima; Adriana Fontes; André A. de Thomas; Carlos L. Cesar

doi:10.1117/12.646912

10 February 2006 Molecular differentiation of Leishmania protozoarium using CdS quantum dots as biolabels

Beate S. Santos, Patrícia M. A. de Farias, Frederico D. de Menezes, Erick L. Mariano, Ricardo de C. Ferreira, Selma Giorgio, Maira C. Bosetto, Diana C. Ayres, Paulo M. Lima, Adriana Fontes, André A. de Thomas, Carlos L. Cesar

Author Affiliations +

Proceedings Volume 6097, Optical Molecular Probes for Biomedical Applications; 609706 (2006) https://doi.org/10.1117/12.646912
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

In this work we applied core-shell CdS/Cd(OH)2 quantum dots (QDs) as fluorescent labels in the Leishmania amazonensis protozoarium. The nanocrystals (8-9 nm) are obtained via colloidal synthesis in aqueous medium, with final pH=7 using sodium polyphosphate as the stabilizing agent. The surface of the particles is passivated with a cadmium hydroxide shell and the particle surface is functionalized with glutaraldehyde. The functionalized and non-functionalized particles were conjugated to Leishmania organisms in the promastigote form. The marked live organisms were visualized using confocal microscopy. The systems exhibit a differentiation of the emission color for the functionalized and non-functionalized particles suggesting different chemical interactions with the promastigote moieties. Two photon emision spectra (λ_exc=795nm) were obtained for the promastigotes labeled with the functionalized QDs showing a significant spectral change compared to the original QDs suspension. These spectral changes are discussed in terms of the possible energy deactivation processes.

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Beate S. Santos, Patrícia M. A. de Farias, Frederico D. de Menezes, Erick L. Mariano, Ricardo de C. Ferreira, Selma Giorgio, Maira C. Bosetto, Diana C. Ayres, Paulo M. Lima, Adriana Fontes, André A. de Thomas, and Carlos L. Cesar "Molecular differentiation of Leishmania protozoarium using CdS quantum dots as biolabels", Proc. SPIE 6097, Optical Molecular Probes for Biomedical Applications, 609706 (10 February 2006); https://doi.org/10.1117/12.646912

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