Direct measurements of blood cell density and velocity in retinal microvessels

F. Lacombe; M. Glanc; J. Le Gargasson; C. Bellmann; M. Paques; J. Sahel

doi:10.1117/12.645241

13 February 2006 Direct measurements of blood cell density and velocity in retinal microvessels

F. Lacombe, M. Glanc, J. Le Gargasson, C. Bellmann, M. Paques, J. Sahel

Author Affiliations +

Proceedings Volume 6138, Ophthalmic Technologies XVI; 61381M (2006) https://doi.org/10.1117/12.645241
Event: SPIE BiOS, 2006, San Jose, California, United States

Abstract

Adaptive Optics assisted eye fundus imaging now provides high resolution images of the very contents of the retinal tissue. Impressive images of the mosaic of the photoreceptors have obtained by several groups and the best reported images even show the circulatory activity in capillars. Nevertheless, the natural eye movements, such as drifts or micro-saccades, generally prevent from an accurate registration of the field of view. Moreover, images obtained through scanning techniques also suffer from field distorsion. These two effects make it difficult to unambiguously extract blood cell density and velocity information. We report how Adaptive Optics assisted flood imaging of the retina, associated with an original a posteriori image registration and data analysis now permits evaluating and mapping the flow activity in the retinal micro-vessels. Examples where capillars as thin as a few micrometers are revealed only by their circulatory activity will be given. Larger field of view along the retinal vascular network will also be produced. Thanks to this high resolution functional mapping, we'll demonstrate how blood cells direct observation can be enhanced and how quantitative parameters such as velocity or density can be derived. Velocity maps of the macular vascular network at the eye's diffraction limit will finally be produced.

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F. Lacombe, M. Glanc, J. Le Gargasson, C. Bellmann, M. Paques, and J. Sahel "Direct measurements of blood cell density and velocity in retinal microvessels", Proc. SPIE 6138, Ophthalmic Technologies XVI, 61381M (13 February 2006); https://doi.org/10.1117/12.645241

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KEYWORDS

Adaptive optics

Capillaries

Eye

Retina

Blood

Imaging systems

Blood vessels

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