A Monte-Carlo study on numerical aperture of lens and size of focal spots in turbid medium

Jie Wang; Zhi-lie Tang; Zu-kang Lu

doi:10.1117/12.674211

9 June 2006 A Monte-Carlo study on numerical aperture of lens and size of focal spots in turbid medium

Jie Wang, Zhi-lie Tang, Zu-kang Lu

Proceedings Volume 6149, 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Advanced Optical Manufacturing Technologies; 61490O (2006) https://doi.org/10.1117/12.674211
Event: 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies, 2005, Xian, China

Abstract

In general, the high resolution of a microscopy could be acquired by increasing the NA (numerical aperture) of lens, and simultaneously increasing the luminous flux. But many experiments shows: in turbid media, the NA of the lens we use is larger, the radius of focal spot is larger and the resolution decreases on the contrary. Because of highly scattering, there are different characters of focusing and propagation in turbid media .We simulate the process of photons motion in turbid media by means of Monte Carlo method according to a known model with representative parameter: changing the NA of lens in the program, the gray-scale images of the light spot in various layer are obtained. We also plot two figures; (1) distribution of the normalized light intensity versus the transverse axis; (2) the radius of light spots as a function of depth. In the figures: the trends of distribution of the normalized light intensity are coincident and centralized; when lens with larger NA is used, the normalized light intensity curve is further from the axis; it is also found the size of the focal spot is bigger and light beams focus in turbid media deeper. All above expound: the NA of the lens is larger; the radius of focal spot is larger in turbid media. In addition, it also suggests various factors including scattering, absorption and scalar diffraction etc must be taken into account when we choose the lens to focus in turbid media.

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Jie Wang, Zhi-lie Tang, and Zu-kang Lu "A Monte-Carlo study on numerical aperture of lens and size of focal spots in turbid medium", Proc. SPIE 6149, 2nd International Symposium on Advanced Optical Manufacturing and Testing Technologies: Advanced Optical Manufacturing Technologies, 61490O (9 June 2006); https://doi.org/10.1117/12.674211

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KEYWORDS

Photons

Tissues

Scattering

Light scattering

Monte Carlo methods

Absorption

Laser scattering

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