Spectral transmission by the specimens of blood-glucose mixture

Elena K. Naumenko; Alexander N. Korolevich

doi:10.1117/12.696991

21 July 2006 Spectral transmission by the specimens of blood-glucose mixture

Elena K. Naumenko, Alexander N. Korolevich

Proceedings Volume 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII; 61630Q (2006) https://doi.org/10.1117/12.696991
Event: Saratov Fall Meeting 2005, 2005, Saratov, Russian Federation

Abstract

Influence of glucose addition to specimens of diluted blood on the transmission spectrums have been investigated by experimentally and by the simulation. The coefficients of extinction, scattering and absorption of erythrocytes were calculated with using Mie theory for spherical particles of equal volume. Transmission spectrums were measured with the spectrophotometer Cary500 in the wavelength range 460-860 nm. Specimens of liquid for imbedding of erythrocytes were preparing by mixing blood plasma and 50-% glucose solution with the different concentrations. The volume concentrations (hematocrit) of red blood cells (RBC) were maintained to have the same values in all specimens by adding equal volume of whole blood to immersion liquid of equal volumes. It has been shown that transmission is decreasing for all wavelengths with the addition of glucose solution on condition that the volume concentrations less than 0.35-0.4. The subsequent increase of the glucose concentration results in increasing of spectral transmission.

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Elena K. Naumenko and Alexander N. Korolevich "Spectral transmission by the specimens of blood-glucose mixture", Proc. SPIE 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, 61630Q (21 July 2006); https://doi.org/10.1117/12.696991

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KEYWORDS

Blood

Glucose

Refractive index

Plasma

Liquids

Absorption

Scattering

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