Optical clearing of human cranial bone by administration of immersion agents

Elina A. Genina; Alexey N. Bashkatov; Valery V. Tuchin

doi:10.1117/12.697308

21 July 2006 Optical clearing of human cranial bone by administration of immersion agents

Elina A. Genina, Alexey N. Bashkatov, Valery V. Tuchin

Proceedings Volume 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII; 616311 (2006) https://doi.org/10.1117/12.697308
Event: Saratov Fall Meeting 2005, 2005, Saratov, Russian Federation

Abstract

We present experimental results on optical properties of cranial bone controlled by administration of propylene glycol and glycerol. Both transmittance and reflectance spectra of human and porcine cranial bone in vitro were measured. Measurements of total transmittance and diffuse reflectance have been performed using a spectrophotometer with an integrating sphere. The reflectance measurements have been carried out with an optical multichannel spectrometer with a fiber-optical probe. For estimation of absorption and reduced scattering coefficients of the bone the inverse adding-doubling method was used. The decrease of reflectance and increase of transmittance of the samples under action of the immersion agents was demonstrated. The experiments have shown that administration of the immersion liquids allows for effective control of tissue optical characteristics, that makes bone more transparent, thereby increasing the ability of light penetration through the tissue. The presented results can be used in developing functional imaging techniques, including OCT and reflectance spectroscopy.

Citation Download Citation

Elina A. Genina, Alexey N. Bashkatov, and Valery V. Tuchin "Optical clearing of human cranial bone by administration of immersion agents", Proc. SPIE 6163, Saratov Fall Meeting 2005: Optical Technologies in Biophysics and Medicine VII, 616311 (21 July 2006); https://doi.org/10.1117/12.697308

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