Optimising a grating-coupled evanescent field excitation

K. Kolari; A. Hokkanen; M. Kuittinen; J. Simonen; P. Heimala

doi:10.1117/12.662622

22 April 2006 Optimising a grating-coupled evanescent field excitation

K. Kolari, A. Hokkanen, M. Kuittinen, J. Simonen, P. Heimala

Proceedings Volume 6189, Optical Sensing II; 61891Z (2006) https://doi.org/10.1117/12.662622
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

Diffraction gratings were designed and fabricated on a SiN/SiO₂ planar waveguide to couple light from a low power 488 nm laser beam into the planar waveguide. The light propagating in the waveguide was then used to detect fluoresceine from volume on the planar waveguide surface. The results demonstrate the capability for very simple and fast analytical throughput for quantification of fluorescent samples, essentially without cross-talk. The transmission measurements show about 10% diffraction efficiency with 0.06° FWHM. The diffraction efficiency and the incidence angle for the maximum diffraction efficiency were observed to be highly dependent on the process parameters used to fabricate the gratings. The fluorescence signal was observed to be linear for fluoresceine concentrations between 10^-9 and 10^-3 M.

Citation Download Citation

K. Kolari, A. Hokkanen, M. Kuittinen, J. Simonen, and P. Heimala "Optimising a grating-coupled evanescent field excitation", Proc. SPIE 6189, Optical Sensing II, 61891Z (22 April 2006); https://doi.org/10.1117/12.662622

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