Protease-activated quantum dot probes

Emmanuel Chang; Jiantang Sun; Jordan S. Miller; William W. Yu; Vicki L. Colvin; Jennifer L. West; Rebekah Drezek

doi:10.1117/12.660485

14 April 2006 Protease-activated quantum dot probes

Emmanuel Chang, Jiantang Sun, Jordan S. Miller, William W. Yu, Vicki L. Colvin, Jennifer L. West, Rebekah Drezek

Proceedings Volume 6191, Biophotonics and New Therapy Frontiers; 61911E (2006) https://doi.org/10.1117/12.660485
Event: SPIE Photonics Europe, 2006, Strasbourg, France

Abstract

We demonstrate a novel quantum dot based probe with inherent signal amplification upon interaction with a targeted proteolytic enzyme. This probe may be useful for imaging in cancer detection and diagnosis. In this system, quantum dots (QDs) are bound to gold nanoparticles (AuNPs) via a proteolytically-degradable peptide sequence to non-radiatively suppress luminescence. A 71% reduction in luminescence was achieved with conjugation of AuNPs to QDs. Peptide cleavage results in release of AuNPs and restores radiative QD photoluminescence. Initial studies observed a 52% rise in luminescence over 47 hours of exposure to 0.2 mg/mL collagenase. These probes can be customized for targeted degradation simply by changing the sequence of the peptide linker.

Citation Download Citation

Emmanuel Chang, Jiantang Sun, Jordan S. Miller, William W. Yu, Vicki L. Colvin, Jennifer L. West, and Rebekah Drezek "Protease-activated quantum dot probes", Proc. SPIE 6191, Biophotonics and New Therapy Frontiers, 61911E (14 April 2006); https://doi.org/10.1117/12.660485

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