Spectral imaging of microscopic samples with high-performance CCD array-based spectrometer

Olga Pawluczyk; Romuald Pawluczyk

doi:10.1117/12.702866

14 February 2007 Spectral imaging of microscopic samples with high-performance CCD array-based spectrometer

Olga Pawluczyk, Romuald Pawluczyk

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 64430G (2007) https://doi.org/10.1117/12.702866
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

The need for parallel spectral analysis of small details in microscopic samples is well recognized in many research fields. Many instruments were proposed for this purpose, some of them using direct projection of an image produced by a standard microscope onto entrance slit of a spectrometer. Typical scanning wavelength spectrometers using focusing reflective gratings have limited imaging performance. These spectrometers also suffer from low light coupling efficiency, poor spatial and spectral resolution, high acquisition times and low image quality. These significant concerns are now addressed by a coupling of a high performance imaging spectrometer to one of the readout ports of a microscope. This spectrometer uses refractive optics, transmission based volume phase holographic (VPH) diffraction gratings and is equipped with two-dimensional array of photodetectors. Such a system provides a significant advantage over most currently used microscope coupled spectrometers, resulting in a larger volume of extracted information, better spectral and spatial resolution, higher SNR and generally better image quality. This is illustrated with examples of spectral images of various biological samples.

Citation Download Citation

Olga Pawluczyk and Romuald Pawluczyk "Spectral imaging of microscopic samples with high-performance CCD array-based spectrometer", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430G (14 February 2007); https://doi.org/10.1117/12.702866

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