Quantitative phase imaging using grating-based quadrature phase interferometer

Jigang Wu; Zahid Yaqoob; Xin Heng; Xiquan Cui; Changhuei Yang

doi:10.1117/12.701694

14 February 2007 Quantitative phase imaging using grating-based quadrature phase interferometer

Jigang Wu, Zahid Yaqoob, Xin Heng, Xiquan Cui, Changhuei Yang

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 64430J (2007) https://doi.org/10.1117/12.701694
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

In this paper, we report the use of holographic gratings, which act as the free-space equivalent of the 3x3 fiber-optic coupler, to perform full field phase imaging. By recording two harmonically-related gratings in the same holographic plate, we are able to obtain nontrivial phase shift between different output ports of the gratings-based Mach-Zehnder interferometer. The phase difference can be adjusted by changing the relative phase of the recording beams when recording the hologram. We have built a Mach-Zehnder interferometer using harmonically-related holographic gratings with 600 and 1200 lines/mm spacing. Two CCD cameras at the output ports of the gratings-based Mach-Zehnder interferometer are used to record the full-field quadrature interferograms, which are subsequently processed to reconstruct the phase image. The imaging system has ~12X magnification with ~420μmx315μm field-of-view. To demonstrate the capability of our system, we have successfully performed phase imaging of a pure phase object and a paramecium caudatum.

Citation Download Citation

Jigang Wu, Zahid Yaqoob, Xin Heng, Xiquan Cui, and Changhuei Yang "Quantitative phase imaging using grating-based quadrature phase interferometer", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 64430J (14 February 2007); https://doi.org/10.1117/12.701694

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KEYWORDS

Phase shifts

Diffraction gratings

Holography

Phase imaging

Beam splitters

Interferometers

Diffraction

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