Fluorescence lifetime imaging using light-emitting diodes

Gordon T. Kennedy; Daniel S. Elson; Ian Munro; Vincent Poher; Paul M. W. French; Mark A. A. Neil

doi:10.1117/12.701088

14 February 2007 Fluorescence lifetime imaging using light-emitting diodes

Gordon T. Kennedy, Daniel S. Elson, Ian Munro, Vincent Poher, Paul M. W. French, Mark A. A. Neil

Author Affiliations +

Proceedings Volume 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV; 644315 (2007) https://doi.org/10.1117/12.701088
Event: SPIE BiOS, 2007, San Jose, California, United States

Abstract

We demonstrate fluorescence lifetime imaging using cheap, high power light emitting diodes as excitation sources. Both time domain and frequency domain fluorescence lifetime imaging techniques have been implemented at wavelengths spanning the range 450 nm to 640 nm.

Citation Download Citation

Gordon T. Kennedy, Daniel S. Elson, Ian Munro, Vincent Poher, Paul M. W. French, and Mark A. A. Neil "Fluorescence lifetime imaging using light-emitting diodes", Proc. SPIE 6443, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XIV, 644315 (14 February 2007); https://doi.org/10.1117/12.701088

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