Laser microtome: all optical preparation of thin tissue samples

Fabian G. Will; Thomas Block; Peggy Menne; Holger Lubatschowski

doi:10.1117/12.713905

13 March 2007 Laser microtome: all optical preparation of thin tissue samples

Fabian G. Will, Thomas Block, Peggy Menne, Holger Lubatschowski

Proceedings Volume 6460, Commercial and Biomedical Applications of Ultrafast Lasers VII; 646007 (2007) https://doi.org/10.1117/12.713905
Event: Lasers and Applications in Science and Engineering, 2007, San Jose, California, United States

Abstract

The laser microtome (LMT) is designed to slice biological tissue and various materials with high precision. The cutting process is performed by an ultrafast laser, emitting infrared light with high repetition rate of about 10 MHz. Biological Tissue can be sliced without fixation and embedding. Therefore cutting of native tissue is possible. Due to the non contact procedure further investigations of the material like immuno histological tests can be performed. At present slices with a thickness of 5 - 100&mgr;m in different biological tissues like cartilage, kidney, lung and cornea has been shown. In conjunction with a 3D imaging system like optical coherence tomography, preparation of 3D tissue volumes is possible too.

Citation Download Citation

Fabian G. Will, Thomas Block, Peggy Menne, and Holger Lubatschowski "Laser microtome: all optical preparation of thin tissue samples", Proc. SPIE 6460, Commercial and Biomedical Applications of Ultrafast Lasers VII, 646007 (13 March 2007); https://doi.org/10.1117/12.713905

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