Optical imaging of TNF-a induced apoptosis pathway in living PC12 cells

Lan Zhang; Da Xing; Miaojuan Chen

doi:10.1117/12.741570

1 May 2007 Optical imaging of TNF-α induced apoptosis pathway in living PC12 cells

Lan Zhang, Da Xing, Miaojuan Chen

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65341C (2007) https://doi.org/10.1117/12.741570
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

Tumor necrosis factor-α (TNF-α) elicits a wide range of biological responses, including neuronal apoptosis and neuroprotection, and this functional pleiotropy is essentially determined by the individual molecular orchestration. Two main pathways lead to apoptosis - the 'extrinsic' or death receptor-initiated pathway, and the 'intrinsic' or mitochondrial pathway. In this study we firstly examine the signaling pathways involved in TNF-α induced apoptosis in living PC12 cells by optical imaging. Our results show that the cleavage of BID has been monitored in real time using fluorescence resonance energy transfer (FRET) technique after PC12 cells treated with TNF-α. Then we observe BAX can't translocation to mitochondria during PC12 cells apoptosis induced by TNF-α, and that there is no any evidence of cytochrome C release into cytosol during cell apoptosis. Our data support that TNF-α mediated PC12 cells apoptosis is extrinsic apoptotic pathway which independent of mitochondria.

Citation Download Citation

Lan Zhang, Da Xing, and Miaojuan Chen "Optical imaging of TNF-α induced apoptosis pathway in living PC12 cells", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341C (1 May 2007); https://doi.org/10.1117/12.741570

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