Light-induced damage and its diagnosis in two-photon excited autofluorescence imaging of retinal pigment epithelium cells

Danni Chen; Junle Qu D.D.S.; Gaixia Xu; Lingling Zhao; Hanben Niu

doi:10.1117/12.741597

1 May 2007 Light-induced damage and its diagnosis in two-photon excited autofluorescence imaging of retinal pigment epithelium cells

Danni Chen, Junle Qu D.D.S., Gaixia Xu, Lingling Zhao, Hanben Niu

Author Affiliations +

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65341L (2007) https://doi.org/10.1117/12.741597
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

In this paper, a novel method for the differentiation of the retinal pigment epithelium (RPE) cells after light-induced damage by two-photon excitation is presented. Fresh samples of RPE cells of pig eyes are obtained from local slaughterhouse. Light-induced damage is produced by the output from Ti: sapphire laser which is focused onto the RPE layer. We study the change of the autofluorescence properties of RPE after two-photon excitation with the same wavelength. Preliminary results show that after two-photon excitation, there are two clear changes in the emission spectrum. The first change is the blue-shift of the emission peak. The emission peak of the intact RPE is located at 592nm, and after excitation, it shifts to 540nm. It is supposed that the excitation has led to the increased autofluorescence of flavin whose emission peak is located at 540nm. The second change is the increased intensity of the emission peak, which might be caused by the accelerated aging because the autofluorescence of RPE would increase during aging process. Experimental results indicate that two-photon excitation could not only lead to the damage of the RPE cells in multiphoton RPE imaging, but also provide an evaluation of the light-induced damage.

Citation Download Citation

Danni Chen, Junle Qu D.D.S., Gaixia Xu, Lingling Zhao, and Hanben Niu "Light-induced damage and its diagnosis in two-photon excited autofluorescence imaging of retinal pigment epithelium cells", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341L (1 May 2007); https://doi.org/10.1117/12.741597

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available