A microarray scanner for the real-time quantitative detection

Quanjun Liu; Ying Zhuang; Lingwei Wu; Zhongwei Wu; Song Hu; Zuhong Lu

doi:10.1117/12.741577

1 May 2007 A microarray scanner for the real-time quantitative detection

Quanjun Liu, Ying Zhuang, Lingwei Wu, Zhongwei Wu, Song Hu, Zuhong Lu

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65341P (2007) https://doi.org/10.1117/12.741577
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

The real-time and quantitative detection assay is important for the gene detection. With the TaqMan probes for the detection based polymerase chain reaction (PCR), four targets could be checked in a single process in solution assay. A new method is developed to immobilize the TaqMan probes on a microarray, which could be used to the multi-target gene fragment quantitative detection with PCR. A new type microarray scanner is designed for the assay. A thermocycler system was built into the scanner platform. A new type of the vessel sealed with the gene amplification solution which could perform the thermo-cycling and scanning. To decrease the background intensity a confocal system was used as the fluorescent intensity detection in the scanner. To calculate the gene quantity, a standard liner graph was draw with the fluorescent intensity versus the cycles. From the standard liner, the quantity of the original gene fragment could be calculated in time with the cycles. This scanner offers the great advantage of real-time quantitative detection of DNA targets in a microarray.

Citation Download Citation

Quanjun Liu, Ying Zhuang, Lingwei Wu, Zhongwei Wu, Song Hu, and Zuhong Lu "A microarray scanner for the real-time quantitative detection", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65341P (1 May 2007); https://doi.org/10.1117/12.741577

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KEYWORDS

Scanners

Luminescence

Target detection

Polymers

Confocal microscopy

Control systems

Optical filters

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