Influencing factors in the measurement of intracellular free calcium

Yuanyuan Li; Sujuan Zhang; Dan Sun; Ying Qu; Xinliang Zheng

doi:10.1117/12.741298

1 May 2007 Influencing factors in the measurement of intracellular free calcium

Yuanyuan Li, Sujuan Zhang, Dan Sun, Ying Qu, Xinliang Zheng

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65342E (2007) https://doi.org/10.1117/12.741298
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

One of the popular methods for measurement of intracellular free calcium ([Ca²⁺]_i) is ratiometric fluorescence technique. R_max and R_min play an important role in the calibration of [Ca²⁺]_i measurement. The purpose of this study was to analyze influencing factors in the [Ca2+]_i measurement. R_max and R_min deduced from theory had a hyperbolic relation with the ratio of C_f and C_b (C_f: free indicator concentration; C_b: Ca²⁺-bound indicator concentration). In experiments, using radiometric fluorescence imaging system, they were measured separately in two types of cell lines in concentration gradient (2×10⁵cells/ml, 5×10⁵cells/ml, 1×10⁶cells/ml and 2×10⁶cells/ml). By Triton method, R_max and R_min of K562 cells were 1.21 and 0.20, respectively, and that of Hepa1-6 cells were 2.02 and 1. These results indicated that R_max and R_min were not influenced by concentration gradient in a certain range of cell concentration, but they were different in various types of cell lines.

Citation Download Citation

Yuanyuan Li, Sujuan Zhang, Dan Sun, Ying Qu, and Xinliang Zheng "Influencing factors in the measurement of intracellular free calcium", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65342E (1 May 2007); https://doi.org/10.1117/12.741298

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