Calcium signaling in UV-induced damage

Dan Sun; Su-juan Zhang; Yuan-yuan Li; Ying Qu; Zhao-Yu Ren

doi:10.1117/12.741301

1 May 2007 Calcium signaling in UV-induced damage

Dan Sun, Su-juan Zhang, Yuan-yuan Li, Ying Qu, Zhao-Yu Ren

Proceedings Volume 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine; 65342F (2007) https://doi.org/10.1117/12.741301
Event: Fifth International Conference on Photonics and Imaging in Biology and Medicine, 2006, Wuhan, China

Abstract

Hepa1-6 cells were irradiated with UV and incubated for varying periods of time. [Ca²⁺]_i (intracellular calcium concentration) of UV-irradiated cell was measured by ratio fluorescence imaging system. The comet assay was used to determine DNA damage. During the UVB-irradiation, [Ca²⁺]_i had an ascending tendency from 0.88 J/m² to 92.4J/m². Comet assay instant test indicated that when the irradiation dosage was above 0.88J/m², DNA damage was observed. Even after approximate 2 h of incubation, DNA damage was still not detected by 0.88J/m² of UVB irradiation. During UVA-irradiation, the elevation of [Ca²⁺]_i was not dose-dependent in a range of 1200 J/m²-6000J/m² and DNA damage was not observed by comet assay. These results suggested that several intracellular UV receptors might induce [Ca²⁺]_i rising by absorption of the UV energy. Just [Ca²⁺]_i rising can't induce DNA damage certainly, it is very likely that the breakdown of calcium steady state induces DNA damage.u

Citation Download Citation

Dan Sun, Su-juan Zhang, Yuan-yuan Li, Ying Qu, and Zhao-Yu Ren "Calcium signaling in UV-induced damage", Proc. SPIE 6534, Fifth International Conference on Photonics and Imaging in Biology and Medicine, 65342F (1 May 2007); https://doi.org/10.1117/12.741301

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