Detection of Bacillus anthracis spores in water using biosensors based on magnetostrictive microcantilever coated with phage

Liling Fu; Suiqiong Li; Kewei Zhang; Z.-Y. Cheng; J.M. Barbaree

doi:10.1117/12.721004

10 May 2007 Detection of Bacillus anthracis spores in water using biosensors based on magnetostrictive microcantilever coated with phage

Liling Fu, Suiqiong Li, Kewei Zhang, Z.-Y. Cheng, J.M. Barbaree

Author Affiliations +

Proceedings Volume 6556, Micro (MEMS) and Nanotechnologies for Defense and Security; 655619 (2007) https://doi.org/10.1117/12.721004
Event: Defense and Security Symposium, 2007, Orlando, Florida, United States

Abstract

Microcantilevers (MCs) as state-of-art sensor platforms have been widely investigated. We recently introduced a new type of MC, magnetostrictive microcantilever (MSMC), as high performance sensor platform. The MSMC is a remote/wireless sensor platform and exhibits a high quality merit factor in liquid. In this paper, a MSMC-based biosensor is developed for detecting B. anthracis spores in liquid, a potential biothreaten agent. The results demonstrated the advantages of MSMCs as a sensor platform. MSMCs with different sizes were fabricated and utilized in the experiments. The MSMCs were coated with the filamentous phage as a bio-recognition element to capture the B. anthracis spores. The phage-coated MSMCs as biosensors were exposed to cultures containing target spores with concentrations ranging from 5 * 10⁴ spores/mL to 5 * 10⁸ spores/mL. The resonance frequency of the MSMC sensors in cultures was monitored in a real-time manner. The results showed that for MSMCs of 2.8 mm * 1.0 mm * 35 &mgr;m and with 1.4 mm * 0.8 mm * 35 &mgr;m have a detection limit of 10⁵ and 10⁴ spores/mL, respectively.

Citation Download Citation

Liling Fu, Suiqiong Li, Kewei Zhang, Z.-Y. Cheng, and J.M. Barbaree "Detection of Bacillus anthracis spores in water using biosensors based on magnetostrictive microcantilever coated with phage", Proc. SPIE 6556, Micro (MEMS) and Nanotechnologies for Defense and Security, 655619 (10 May 2007); https://doi.org/10.1117/12.721004

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