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Tissue specific progenitor cells and its differentiations have got a lot of attentions in regenerative medicine. The process of
differentiations, the formation of tissues, has become better understood by the study using a lot of cell types progressively.
These studies of cells and tissue dynamics at molecular levels are carried out through various approaches like histochemical
methods, application of molecular biology and immunology. However, in case of using regenerative sources (cells, tissues
and biomaterials etc.) clinically, they are measured and quality-controlled by non-contact and non-destructive methods
from the view point of safety. Or the analysis with small quantities of materials could be possible if the quantities of
materials are acceptable. A non-contact and non-destructive quality control method has been required. Recently, the use of
Fourier Transform Infrared spectroscopy (FT-IR) has been used to monitor biochemical changes in cells, and has gained
considerable importance. The changes in the cells and tissues, which are subtle and often not obvious in the
histpathological studies, are shown to be well resolved using FT-IR. Moreover, although most techniques designed to
detect one or a few changes, FT-IR is possible to identify the changes in the levels of various cellular biochemicals
simultaneously under in vivo and in vitro conditions. The objective of this study is to establish the infrared spectroscopy of
tissue specific progenitor cell differentiations as a quality control of cell sources for regenerative medicine. In the present
study, as a basic study, we examine the adipose differentiation kinetics of preadipose cells (3T3-L1) and the osteoblast
differentiation kinetics of mesenchymal stem cells (Kusa-A1) to analyze the infrared absorption spectra.
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