Characterization of silver nanoparticles deposited by an enzyme

Thomas Schüler; Robert Möller; Andrea Steinbrück; Wolfgang Fritzsche; Jürgen Popp

doi:10.1117/12.728138

13 July 2007 Characterization of silver nanoparticles deposited by an enzyme

Thomas Schüler, Robert Möller, Andrea Steinbrück, Wolfgang Fritzsche, Jürgen Popp

Proceedings Volume 6633, Biophotonics 2007: Optics in Life Science; 66331V (2007) https://doi.org/10.1117/12.728138
Event: European Conferences on Biomedical Optics, 2007, Munich, Germany

Abstract

Based on their various interesting properties metal nanoparticles show the potential as analytical tool in electronic, optical, and catalytical applications. The different properties depending on composition, shape, and size of the single particles were utilized in many different approaches such as optics, magnetics and laser technology¹. We present a way for enzymatic deposition of silver nanoparticles and a bioanalytical application in DNA microarray technology for this method. The technology consists of a microstructured chip with 10&mgr;m broad electrode gaps on the surface and specially designed readout device². In principle we immobilize gold nanoparticle-labelled DNA in a gap between two electrodes. Afterwards a silver deposition on the bound gold nanoparticles generates a conductive layer between the electrodes. The measured drop in the resistance serves as signal for the chip-based electrical detection of DNA³. To further optimize this system the gold nanoparticles as seed are replaced by the enzyme horseradish peroxidase. For a better understanding of the enzymatically silver deposition process the formed silver particles were analyzed by spectroscopic characterization on a single particle level. Further investigations of these particles by AFM and SEM should give a hint to the connection between size/shape and the plasmonic properties at individual particles.

Citation Download Citation

Thomas Schüler, Robert Möller, Andrea Steinbrück, Wolfgang Fritzsche, and Jürgen Popp "Characterization of silver nanoparticles deposited by an enzyme", Proc. SPIE 6633, Biophotonics 2007: Optics in Life Science, 66331V (13 July 2007); https://doi.org/10.1117/12.728138

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