Optical phase contrast microscope imaging: a FDTD modeling approach

Stoyan Tanev; James Pond; Paul Paddon; Valery V. Tuchin

doi:10.1117/12.803973

9 June 2008 Optical phase contrast microscope imaging: a FDTD modeling approach

Stoyan Tanev, James Pond, Paul Paddon, Valery V. Tuchin

Proceedings Volume 6791, Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX; 67910E (2008) https://doi.org/10.1117/12.803973
Event: Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX, 2006, Saratov, Russian Federation

Abstract

The Finite-Difference Time-Domain (FDTD) modeling technique is applied to build a simple simulation equivalent of an optical phase contrast microscope. The model is validated by demonstrating the effect of optical immersion on the optical phase contrast microscope image of a simple biological cell containing a cytoplasm, a nucleus and a membrane. To the best of our knowledge, this is the first study using the FDTD approach to construct optical phase contrast microscope images. The results demonstrate the potential of the FDTD modelling approach and extend its area of applicability a new biomedical research area.

Citation Download Citation

Stoyan Tanev, James Pond, Paul Paddon, and Valery V. Tuchin "Optical phase contrast microscope imaging: a FDTD modeling approach", Proc. SPIE 6791, Saratov Fall Meeting 2007: Optical Technologies in Biophysics and Medicine IX, 67910E (9 June 2008); https://doi.org/10.1117/12.803973

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