Methods on observation of fluorescence micro-imaging for microalgae

Lin Ou; Hui-ru Zhuang; Rong Chen; Jin-pin Lei; Xiao-hua Liao; Wen-suo Lin

doi:10.1117/12.756970

11 January 2008 Methods on observation of fluorescence micro-imaging for microalgae

Lin Ou, Hui-ru Zhuang, Rong Chen, Jin-pin Lei, Xiao-hua Liao, Wen-suo Lin

Author Affiliations +

Proceedings Volume 6826, Optics in Health Care and Biomedical Optics III; 68262P (2008) https://doi.org/10.1117/12.756970
Event: Photonics Asia 2007, 2007, Beijing, China

Abstract

Objective: Auto-fluorescence micro-imaging of microalgae are observed by using of laser scanning confocal microscopy (LSCM) and fluorescence microscopy, so as to investigate the effect of auto fluorescence alteration on growth of irradiated microalgae irradiated, meanwhile, the method of microalgae cells stained also to be studied. Methods: Platymonas subcordiformis, Phaeodactylum tricormutum and Isochyrsis zhanjiangensis cells are stained with acridine orange, and observed by fluorescence microscopy; the three types microalgae mentioned above are irradiated by Nd:YAP laser with 10w at 1341nm, irradiating time:12s, 30s, 35s and 55s, than to be cultured 6 days, and the auto fluorescence images and fluorescence spectra of algae cells are obtained by LSCM on lambda scan mode, at excitation 488nm (Ar⁺ laser). Results: It is showed that the shapes and the structural features of microalgae cells stained can be seen clearly, and the cytoplasm and nucleus also can be observed. The chloroplasts in cell is bigger on promoting effects, conversely, it is to be mutilated, deformation and shrink. Contrast to the CK, the peak positions of fluorescence of algae cells irradiated is similar to the whole while the peak light intensity alters. On irradiation of promoting dose, however, the auto fluorescence intensity is enhanced more than control. Conclusions: The method of cell stained can be used to observed genetic material in microalgae. There are obvious effects for laser irradiating to chloroplasts in cells, the bigger chloroplasts the greater fluorescence intensity. Physiological incentive effects of microalgae irradiated can be given expression on fluorescence characteristics and fluorescence intensity alteration of cells.

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Lin Ou, Hui-ru Zhuang, Rong Chen, Jin-pin Lei, Xiao-hua Liao, and Wen-suo Lin "Methods on observation of fluorescence micro-imaging for microalgae", Proc. SPIE 6826, Optics in Health Care and Biomedical Optics III, 68262P (11 January 2008); https://doi.org/10.1117/12.756970

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KEYWORDS

Luminescence

Oceanography

Microscopes

Confocal microscopy

Argon

Microscopy

Genetics

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