Fluorescence quantum yield of verteporfin is independent of oxygen

Tim Monahan; Shudong Jiang; Brian Pogue

doi:10.1117/12.764244

27 February 2008 Fluorescence quantum yield of verteporfin is independent of oxygen

Tim Monahan, Shudong Jiang, Brian Pogue

Proceedings Volume 6845, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII; 684517 (2008) https://doi.org/10.1117/12.764244
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Photodynamic therapy dosimetery and treatment planning is affected by the concentration of photosensitizer in a given tissue, and these values are often estimated based on measurements of fluorescence in the region to be treated. Some studies with benzoporphyrin derivate monoacid ring a (BPD-MA) showed a significant increase in fluorescence quantum yield with deoxygenation of the solution, indicating a possible oxygen sensitive switch in intersystem crossing or reverse intersystem crossing. The experiments done in this paper show that at oxygenation levels found in vivo the variation in fluorescence quantum yield of liposomal BPD-MA (verteporfin) is negligible for changes in solution oxygenation. The results from all of the experiments show that it is not necessary to measure the oxygenation of tissues when calculating the concentration of verteporfin from fluorescence measurements, so that dosimetry calculations based upon photosensitizer levels would not be affected by the tissue oxygenation. This greatly simplifies the dosimetry process with verteporfin.

Citation Download Citation

Tim Monahan, Shudong Jiang, and Brian Pogue "Fluorescence quantum yield of verteporfin is independent of oxygen", Proc. SPIE 6845, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy XVII, 684517 (27 February 2008); https://doi.org/10.1117/12.764244

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