Raman spectroscopic study of a genetically altered kidney cell

Joel Joshi; Francisco Garcia; Silvia P. Centeno; N. V. Joshi

doi:10.1117/12.760317

5 February 2008 Raman spectroscopic study of a genetically altered kidney cell

Joel Joshi, Francisco Garcia, Silvia P. Centeno, N. V. Joshi

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 685304 (2008) https://doi.org/10.1117/12.760317
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

A Raman spectroscopic investigation of a genetically altered Human Embryonic Kidney Cell (HEK293) along with a pathologically normal cell has been carried out by a conventional method. The genetic alteration was carried out with a standard protocol by using a Green Fluorescence Protein (GFP). Raman spectra show that there are dramatic differences between the spectrum obtained from a genetically altered cell and that obtained from a pathologically normal cell. The former shows three broad bands; meanwhile the latter shows several sharp peaks corresponding to the ring vibrational modes of Phen, GFP and DNA. The present analysis provides an indication that the force field near Phen located at 64, 65 and 66 was altered during the genetic transformation. The Raman spectrum could be a direct experimental evidence for substantial modifications triggered due to the expression of specific genes.

Citation Download Citation

Joel Joshi, Francisco Garcia, Silvia P. Centeno, and N. V. Joshi "Raman spectroscopic study of a genetically altered kidney cell", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685304 (5 February 2008); https://doi.org/10.1117/12.760317

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