A comparative study of intrinsic versus bulk polarized fluorescence in cervical tissues

Dharitri Rath; Md. Ejaz A. Lodhi; Prashant Shukla; Nidhi Agarwal; Kiran Pandey; Asima Pradhan

doi:10.1117/12.763420

8 February 2008 A comparative study of intrinsic versus bulk polarized fluorescence in cervical tissues

Dharitri Rath, Md. Ejaz A. Lodhi, Prashant Shukla, Nidhi Agarwal, Kiran Pandey, Asima Pradhan

Proceedings Volume 6853, Biomedical Optical Spectroscopy; 685319 (2008) https://doi.org/10.1117/12.763420
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

This study aims towards applying the intrinsic polarized fluorescence technique for obtaining valuable information from human cervical tissue samples. The efficacy of this technique is tested in human tissues by comparing its diagnostic capabilities with the bulk fluorescence. It is seen that biochemical information is hidden due to the presence of distortions by tissue scattering and absorption in the fluorescence spectra. Intrinsic tissue fluorescence provides a complete understanding of the biochemical and/or morphological changes that take place during the progression of disease. Recording of the experimental data and thereafter subsequent extraction of the intrinsic fluorescence serves as fingerprints towards determining the occurrence of these diseases. Here we report a comparative study of intrinsic versus bulk polarized fluorescence in cervical tissues. Intrinsic fluorescence is seen to be a more sensitive technique than bulk fluorescence for diagnosis of cervical cancers. Attempts have been made to study the changes in the amount of different fluorophores found in the epithelial and stromal layer of cervical tissue (both normal and cancerous). It has been seen that collagen decreases and NADH increases as a healthy cervical tissue develops into a cancerous one. Intrinsic fluorescence provides more consistent discriminating results as compared to bulk polarized fluorescence. It is also more sensitive in giving biochemical information from the different layers in cervical tissue. It may be concluded that intrinsic fluorescence shows promise as a viable tool for providing valuable insights towards fruitful diagnosis of the various stages of disease development and changes occurring with age.

Citation Download Citation

Dharitri Rath, Md. Ejaz A. Lodhi, Prashant Shukla, Nidhi Agarwal, Kiran Pandey, and Asima Pradhan "A comparative study of intrinsic versus bulk polarized fluorescence in cervical tissues", Proc. SPIE 6853, Biomedical Optical Spectroscopy, 685319 (8 February 2008); https://doi.org/10.1117/12.763420

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