Modification of measurement methods for evaluation of tissue-engineered cartilage function and biochemical properties using nanosecond pulsed laser

Miya Ishihara; Masato Sato M.D.; Toshiharu Kutsuna M.D.; Masayuki Ishihara; Joji Mochida M.D.; Makoto Kikuchi D.D.S.

doi:10.1117/12.762481

6 February 2008 Modification of measurement methods for evaluation of tissue-engineered cartilage function and biochemical properties using nanosecond pulsed laser

Miya Ishihara, Masato Sato M.D., Toshiharu Kutsuna M.D., Masayuki Ishihara, Joji Mochida M.D., Makoto Kikuchi D.D.S.

Author Affiliations +

Proceedings Volume 6858, Optics in Tissue Engineering and Regenerative Medicine II; 685805 (2008) https://doi.org/10.1117/12.762481
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

There is a demand in the field of regenerative medicine for measurement technology that enables determination of functions and components of engineered tissue. To meet this demand, we developed a method for extracellular matrix characterization using time-resolved autofluorescence spectroscopy, which enabled simultaneous measurements with mechanical properties using relaxation of laser-induced stress wave. In this study, in addition to time-resolved fluorescent spectroscopy, hyperspectral sensor, which enables to capture both spectral and spatial information, was used for evaluation of biochemical characterization of tissue-engineered cartilage. Hyperspectral imaging system provides spectral resolution of 1.2 nm and image rate of 100 images/sec. The imaging system consisted of the hyperspectral sensor, a scanner for x-y plane imaging, magnifying optics and Xenon lamp for transmmissive lighting. Cellular imaging using the hyperspectral image system has been achieved by improvement in spatial resolution up to 9 micrometer. The spectroscopic cellular imaging could be observed using cultured chondrocytes as sample. At early stage of culture, the hyperspectral imaging offered information about cellular function associated with endogeneous fluorescent biomolecules.

Citation Download Citation

Miya Ishihara, Masato Sato M.D., Toshiharu Kutsuna M.D., Masayuki Ishihara, Joji Mochida M.D., and Makoto Kikuchi D.D.S. "Modification of measurement methods for evaluation of tissue-engineered cartilage function and biochemical properties using nanosecond pulsed laser", Proc. SPIE 6858, Optics in Tissue Engineering and Regenerative Medicine II, 685805 (6 February 2008); https://doi.org/10.1117/12.762481

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