Tissue morphology from spectral polarimetry

Donald D. Duncan; James C. Gladdish; Brandon D. Markway

doi:10.1117/12.760512

6 February 2008 Tissue morphology from spectral polarimetry

Donald D. Duncan, James C. Gladdish, Brandon D. Markway

Proceedings Volume 6858, Optics in Tissue Engineering and Regenerative Medicine II; 685809 (2008) https://doi.org/10.1117/12.760512
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

An important issue in the development of cultured tissues is the alignment of the cells within the scaffold, or on the substrate. Proper alignment leads to optimum tissue strength and it has been demonstrated that proper alignment is engendered by application of physiologically realistic stresses during the cell proliferation process. In situ monitoring of cell alignment during development can provide important feedback information in determining the optimum stresses. Numerical calculations suggest that cell aspect and orientation can be inferred from the polarization of the light scattered by these cells. In this paper, we demonstrate that a measurement of the wavelength-dependent depolarization of the light scattered from the cell layer reveals the alignment of these cells. We present results of experimental measurements on human umbilical vein endothelial cells (HUVEC's) layered onto glass cover slips and of simulations using T-matrix methods.

Citation Download Citation

Donald D. Duncan, James C. Gladdish, and Brandon D. Markway "Tissue morphology from spectral polarimetry", Proc. SPIE 6858, Optics in Tissue Engineering and Regenerative Medicine II, 685809 (6 February 2008); https://doi.org/10.1117/12.760512

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