Single molecules in fluorescence fluctuation spectroscopy: effective volume and photon counting histogram

Jörg Ackermann; Harald Mathis; Benjamin Greiner

doi:10.1117/12.763904

21 February 2008 Single molecules in fluorescence fluctuation spectroscopy: effective volume and photon counting histogram

Jörg Ackermann, Harald Mathis, Benjamin Greiner

Author Affiliations +

Proceedings Volume 6862, Single Molecule Spectroscopy and Imaging; 686204 (2008) https://doi.org/10.1117/12.763904
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

This paper presents a stochastic theory for the interpretation of photon counting histograms in fluorescence fluctuation spectroscopy (FFS). New concepts of an effective volume and a single molecule probability distribution are introduced to characterize a molecular species. Whereas the effective volume corresponds to the visibility of a molecular species in a given confocal setup, the single molecule probability distribution gives the signal measured for a single visible molecule. Specific properties of the effective volume and the single molecule probability distribution are discussed. Advantages arise for the high precision measurements of concentrations, mixtures, and binding constants especially for complex molecular environment, e.g. in flow systems and cell compartments.

Citation Download Citation

Jörg Ackermann, Harald Mathis, and Benjamin Greiner "Single molecules in fluorescence fluctuation spectroscopy: effective volume and photon counting histogram", Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 686204 (21 February 2008); https://doi.org/10.1117/12.763904

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