Fluorescence microscope using total internal reflection

Soo Jin Bae; Seung Yup Lee; Uk Kang

doi:10.1117/12.764347

21 February 2008 Fluorescence microscope using total internal reflection

Soo Jin Bae, Seung Yup Lee, Uk Kang

Proceedings Volume 6862, Single Molecule Spectroscopy and Imaging; 686210 (2008) https://doi.org/10.1117/12.764347
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

In this experimental investigation, we suggest a new fluorescence microscope and method using a total internal reflection fluorescence. Unlike the conventional a total internal reflection fluorescence scheme, it uses fluorescence obtained due to the excitation due to the excitation of micro-objects occurring over the entire thickness of a sample, not a region in which an evanescent field extends. By the proposed scheme, the observation of the fluorescence of a biological micochip is possible without the installation of optical filters on excitation and reception light paths. It is possible to observe a small amount of fluorescent dye due to the reduction of the occurrence of optical noises and to simultaneously observe fluorescence images emitted from various fluorescent dyes without optical filters, so that its construction become simple and the size decreases.

Citation Download Citation

Soo Jin Bae, Seung Yup Lee, and Uk Kang "Fluorescence microscope using total internal reflection", Proc. SPIE 6862, Single Molecule Spectroscopy and Imaging, 686210 (21 February 2008); https://doi.org/10.1117/12.764347

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