Combining AFM and FRET for studies at the cellular level

Kenith E. Meissner; Zhe Sun; Bhavik Nathwani; Clark Needham; Richard E. Beckham; William N. Everett; Xudong Fan; Gerard L. Cote; Gerald A. Meininger

doi:10.1117/12.769175

22 February 2008 Combining AFM and FRET for studies at the cellular level

Kenith E. Meissner, Zhe Sun, Bhavik Nathwani, Clark Needham, Richard E. Beckham, William N. Everett, Xudong Fan, Gerard L. Cote, Gerald A. Meininger

Author Affiliations +

Proceedings Volume 6863, Optical Diagnostics and Sensing VIII; 686302 (2008) https://doi.org/10.1117/12.769175
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

We report on the development of a Forster resonance energy transfer (FRET) enabled atomic force microscope (AFM) for the study of biomechanics and mechanobiology at the cellular level. The hybrid microscopy system combines the spatial resolution and control of the AFM with the nanoscale sensing capabilities of FRET to enable simultaneous detection of cell mechanical responses and correlation of those responses with cellular biochemistry. Here, we show FRET signal from donor-coated microspheres, that are attached to an AFM cantilever, to acceptor-labeled integrins in a fixed cell system. Additionally, we demonstrate and discuss the attachment of quantum dots to silica microspheres as the FRET donor.

Citation Download Citation

Kenith E. Meissner, Zhe Sun, Bhavik Nathwani, Clark Needham, Richard E. Beckham, William N. Everett, Xudong Fan, Gerard L. Cote, and Gerald A. Meininger "Combining AFM and FRET for studies at the cellular level", Proc. SPIE 6863, Optical Diagnostics and Sensing VIII, 686302 (22 February 2008); https://doi.org/10.1117/12.769175

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
9 PAGES

DOWNLOAD PAPER SAVE TO MY LIBRARY

GET CITATION

RIGHTS & PERMISSIONS

Get copyright permission Get copyright permission on Copyright Marketplace

KEYWORDS

Fluorescence resonance energy transfer

Quantum dots

Atomic force microscopy

Luminescence

Silica

Absorption

Epoxies

Show All Keywords

Keywords/Phrases

Search In:

Publication Years