Non-invasive monitoring and quantitative analysis of patch test reactions by reflectance spectrophotometry, laser Doppler flowmetry and transepidermal water loss

Natalja Skrebova Eikje M.D.; Seiji Arase

doi:10.1117/12.765816

22 February 2008 Non-invasive monitoring and quantitative analysis of patch test reactions by reflectance spectrophotometry, laser Doppler flowmetry and transepidermal water loss

Natalja Skrebova Eikje M.D., Seiji Arase

Author Affiliations +

Proceedings Volume 6864, Biomedical Applications of Light Scattering II; 68640E (2008) https://doi.org/10.1117/12.765816
Event: SPIE BiOS, 2008, San Jose, California, United States

Abstract

Reflectance spectrophotometry (RS), laser Doppler flowmetry (LDF) and transepidermal water loss (TEWL) techniques were simultaneously used to non-invasively monitor skin colour (SC), skin blood flow (SBF) and barrier function damage (BFD) in routinely patch-tested Japanese patients in dermatology clinic. The analytical quality, reliability and reproducibility of each technique were compared and analyzed in correlated to visual scoring patch test (PT) reactions as negative (-), doubtful (+?), weak (+) and strong (++/+++) at 48- and 72-hour monitoring. An attempt was made to quantify predominant in the clinic "+?"- and "+'"-PT-reactions. The relationship between 48 h and 72 h measurements in different reaction groups was poor for TEWL, LDF showed a tendency to decrease at 72 h, but good for RS. A correlation between visual scorings and instrumental mean values was poor for TEWL, good for LDF and excellent for RS. So, measurements by RS were the most statistically significant to non-invasively monitor and quantify doubtful, weak and strong PT reactions, accordingly providing continuous data grading of reaction intensity suitable in the clinic. Moreover, monitoring of SC changes was the most reliable parameter for the quantitative distinguishing of doubtful and weak reactions in pigmented skin.

Citation Download Citation

Natalja Skrebova Eikje M.D. and Seiji Arase "Non-invasive monitoring and quantitative analysis of patch test reactions by reflectance spectrophotometry, laser Doppler flowmetry and transepidermal water loss", Proc. SPIE 6864, Biomedical Applications of Light Scattering II, 68640E (22 February 2008); https://doi.org/10.1117/12.765816

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